FastQCFastQC Report
Mon 27 Feb 2023
SRR3128855.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128855.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2305820
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179160.7769903982097476No Hit
CTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGC34110.1479300205566783No Hit
CCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTG32570.14125126852920003No Hit
GCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTG26710.11583731600905535No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT69700.082.505781
TAGGGCA11250.076.866384
AGGGATG36100.075.6385
GAATAGG15250.074.369211
ATAGGGC14050.073.924273
ATAGGGA21400.072.472173
GAGGGAT29050.071.3454
AGGGAAT22400.071.1250845
CGTAGGG6750.071.064072
GTAGGGC9600.069.516553
AAGGGAT36550.069.4348454
AGTAGGG36500.069.3175352
AGTAAGG15550.069.302841
TATAGGG19250.069.1368262
TAAGGGA20050.068.679063
CGAGGGA13200.068.359433
ACGGGAT8600.068.3097464
ATGTAGG6350.068.180481
TAGGGTA5950.067.928594
AGGGCAT21050.067.2025155
Produced by FastQC (version 0.11.5)