FastQCFastQC Report
Mon 27 Feb 2023
SRR3128854.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128854.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2411463
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183230.7598291991210315No Hit
CTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGC38560.15990293029584116No Hit
CCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTG35790.14841612747116584No Hit
GCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTG30440.126230425264663No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT71500.081.060081
CGTAGGG7700.076.295372
ATAGGGA21800.075.4554143
AGGGATG39700.074.576495
GAATAGG15650.072.745041
GTAGGGA16700.070.919063
GAGGGAT30800.070.191744
AAGGGAT40050.070.056874
TAGGGCG3700.069.861824
AGTAAGG14450.069.67041
TAAGGGA21050.069.659673
ATAGGGC12950.069.317443
TAATAGG8350.069.298051
TAGGGAT18500.068.845644
TAGGGAC12400.068.6018454
TAGGGCA10350.068.567054
AAGGGTA17950.068.3368454
GTAGGGC10400.067.78553
TATAGGG18300.067.5434952
AGTTAGG6150.067.31471
Produced by FastQC (version 0.11.5)