FastQCFastQC Report
Mon 27 Feb 2023
SRR3128853.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128853.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1971484
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT425182.156649508694973No Hit
CCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTG45240.2294718090534846No Hit
CGCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCT42180.2139505063190977No Hit
CGTTCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTT41650.21126217610693263No Hit
CGTTTCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCT36500.1851397221585364No Hit
CTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC33460.1697198658472501TruSeq Adapter, Index 13 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT160800.087.603321
TAGGGCA9900.075.714414
AGGGATG29100.072.993325
GTAGGGC9550.070.5909963
TAGAGGG33550.069.97672
AGAGGGC30050.069.34173
AGTAGGG29700.069.206462
ATAGGGC11100.068.80323
AAGAGGG67500.067.466782
ACGGGTA2800.067.347054
TATAGGG14150.067.299452
TAGGGTA6250.067.1315464
ATAGAGG13700.067.1251751
AAGGGTA13900.066.814094
GAGGGTA10050.066.140844
GAATAGG12650.065.613541
CGTAGGG5400.065.47632
GTAGGGA15200.065.131693
AAGGGAT31500.065.102154
AGTAAGG13300.064.888961
Produced by FastQC (version 0.11.5)