FastQCFastQC Report
Mon 27 Feb 2023
SRR3128852.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128852.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2022589
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT425212.102305510412644No Hit
CCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTG48240.2385061918165282No Hit
CGCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCT44890.2219432618292693No Hit
CGTTCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTT44010.21759240260873564No Hit
CTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC38380.18975679191373038TruSeq Adapter, Index 13 (95% over 21bp)
CGTTTCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCT38260.18916349292911214No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT166450.087.170181
AGGGATG28200.072.3296055
GTAGGGC10350.070.382843
AGTAAGG12900.069.6596151
ACGGGTA2600.068.688774
ATAGGGC11400.068.435073
TAGAGGG34500.067.321712
ATAGGGA17550.066.144743
TAGGGCA9250.066.0506444
TATAGGG15500.065.822642
AGAGGGC31000.065.796613
ATAGAGG13900.065.663541
AGTAGGG32050.065.426562
AAGGGAT30100.065.4218754
TACGGGT1800.065.2744143
GAATAGG10400.065.1428151
AAGAGGG70450.064.868312
TAGTAGG8850.064.856561
AGAGGGA44000.064.621673
AGGGACT17700.063.991055
Produced by FastQC (version 0.11.5)