FastQCFastQC Report
Mon 27 Feb 2023
SRR3128851.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128851.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2103204
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT434372.0652775479696692No Hit
CCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTG51760.24610071110553233No Hit
CGCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCT46430.22075842381433278No Hit
CGTTCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTT45530.2164792383430233No Hit
CTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC38960.18524118440246407TruSeq Adapter, Index 13 (95% over 21bp)
CGTTTCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCT38690.1839574287610712No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT165650.086.723311
ATAGCGG4000.076.463451
ACGGGTA2250.075.19584
AGGGATG30900.072.545895
GTAGGGC10400.071.399863
ATAGGGC11500.070.2917253
ATAGGGA18350.069.91983
AGAGGGC32000.069.321133
AGTAGGG34100.068.7716452
AGATAGG11450.068.62961
GAATAGG13450.068.570021
AGGGTAC8750.067.6735
TATAGGG14900.067.182562
GAGGGTA12600.067.139114
AAGGGAT32100.066.46984
TAAGGGA16650.066.050363
AAGAGGG75800.065.906362
TAGGGTA6500.065.796324
AGTAAGG13600.065.73781
TAGAGGG37200.065.6936952
Produced by FastQC (version 0.11.5)