FastQCFastQC Report
Mon 27 Feb 2023
SRR3128850.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128850.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2333316
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT243931.04542205170667No Hit
CCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTG27370.11730087137790167No Hit
CTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC26720.11451513639815611No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT93750.084.162061
ATAGGGC12650.075.6987463
GTAGGGC10800.075.562363
GTACGGG5450.074.436362
TAGGGCA13250.074.050954
AGGGATG33250.071.795235
AGGGTAC12150.071.1647645
TAGACGG2400.070.785241
ATAGCGG4800.069.802111
ATAGAGG15800.068.99321
ATAGGGA19100.068.4115753
AGTACGG3800.068.301541
TAGAGGG42800.068.002462
GAATAGG13950.067.9944151
AGGGCAT19100.067.658575
AATAGGG35150.067.235032
AGAGGGC33400.066.9445343
TAAGGGA19150.066.262343
CGTAGGG7000.066.0406342
AGTAGGG38450.066.003832
Produced by FastQC (version 0.11.5)