FastQCFastQC Report
Mon 27 Feb 2023
SRR3128849.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128849.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2385620
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT239661.0046025771078377No Hit
CCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTG30210.12663374720198523No Hit
CTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC29340.12298689648812468No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT97100.081.40911
TAGACGG3200.073.519561
ATAGGGC12250.072.893763
GTAGGGC9000.072.062523
GAATAGG12600.068.711621
AGGGTAC12600.068.6309745
TAGAGGG43750.068.577172
AAGGGTA18950.068.44994
TAAGGGA18700.068.35973
AGGGATG32350.067.554085
AGTAGGG35550.067.463292
ACGGGAT7000.067.138994
TGTAGGG16400.067.09792
AGGGTAG13950.067.042745
AGTAAGG15150.066.774211
TAAGGGT11450.066.4939963
GAGGGTA17100.066.2359544
ATAAGGG33950.066.210222
ATAGCGG5200.066.0545041
ATAGGGA19500.066.0372243
Produced by FastQC (version 0.11.5)