FastQCFastQC Report
Mon 27 Feb 2023
SRR3128848.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128848.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2488270
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT246910.9922958521382326No Hit
CCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTG32370.13009038408211326No Hit
CTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC31480.12651360181973823No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT99200.081.012811
ATAGGGC13500.074.847233
GGTAAGG16050.072.116991
GTAGGGC11650.070.99993
TAGAGGG45750.070.571272
TAGGGCA14150.070.0804754
ATAGGGA20550.068.1515053
AGGGATG34000.068.004835
AGGGTAC14250.066.9475565
AGTAGGG42400.066.503982
GTACGGG6600.066.221852
CGTAGGG7600.066.1656342
TAGGGAT14500.065.795934
AATAGGG36900.065.718192
TATAGGG21050.065.638332
TAGGGTC7900.065.4389954
TAAGGGA19050.065.376533
AAGGGGC81750.065.364774
ATAAGGG35850.065.2833562
AAGAGGG96200.065.2181552
Produced by FastQC (version 0.11.5)