FastQCFastQC Report
Mon 27 Feb 2023
SRR3128847.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128847.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2124484
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133530.6285290922407512No Hit
CTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC32640.1536373067530751No Hit
CCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTG31800.14968340547634154No Hit
GCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTG28350.13344416808975731No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT56700.075.559831
ATAGGGC14050.074.1890643
ATAACGG2450.071.2563861
GGTAAGG13500.069.901161
TAGGGCA11600.069.121784
AGGGATG31800.068.8645255
AAGGGTA18650.068.7882164
CGTAGGG7700.068.6042942
AAGGGAT39050.068.241944
GAATAGG11550.067.813211
AGGGTAC11100.067.738215
TAGAGGG38150.067.379222
TAACGGG7650.067.2030642
AAGGGGC64850.067.202584
ATAAGGG32750.067.1117552
AGGGCAT19800.066.929985
AGTAGGG36350.066.823132
AGGGTAT15300.066.650765
ATAGGGT10950.066.3331453
GTAGGGT9450.065.88193
Produced by FastQC (version 0.11.5)