FastQCFastQC Report
Mon 27 Feb 2023
SRR3128846.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128846.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2198152
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130780.5949543070724863No Hit
CTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC37440.17032489109033408No Hit
CCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTG33260.15130891767266322No Hit
GCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTG29730.13524997361419958No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT62300.076.510011
AAGGGTA20450.072.391944
ATAGGGC15200.072.351113
AGGGTAC11850.070.991715
AGGGATG34900.069.889975
AAGGGAT36050.068.5730444
GGTAAGG13100.068.246311
TAGGGTA7950.067.983524
TAGAGGG41100.067.967952
GAATAGG12100.067.27561
AGAGGGC31450.066.946873
AGGGCAT19100.066.928095
TAGGGCA10350.066.74984
TAGGGAT16950.066.267584
TACGGGT1850.066.050263
AAGAGGG78850.065.547412
GTAGGGC9000.065.274033
TAAGAGG17350.065.089151
ATAGGGT10350.064.933483
ATAAGGG31950.064.912342
Produced by FastQC (version 0.11.5)