FastQCFastQC Report
Mon 27 Feb 2023
SRR3128845.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128845.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2293515
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136470.595025539401312No Hit
CTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC39200.1709166933724No Hit
CCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTG34410.15003171987102767No Hit
GCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTG31190.13599213434400909No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT60900.076.5101241
ATAGGGC12850.075.701583
CGTAGGG7000.074.518252
TAGGGTA8300.071.3395164
GAATAGG12500.070.409711
AAGGGTA19950.069.489124
AGGGTAC11300.068.618825
TAGTAGG12450.068.424271
AGGGATG35900.067.544965
TAGGGCA11000.067.499744
AGTAGGG39900.067.2513352
GGTAAGG12550.067.129021
ATAGGGT13100.067.082313
TAGAGGG40750.066.65582
CGAAGGG22350.066.44272
TAAGAGG18050.066.230421
AATAGGG34350.066.214992
AGGGCAT21150.065.990865
AAGAGGG90550.065.9102552
AGATAGG14850.065.60621
Produced by FastQC (version 0.11.5)