FastQCFastQC Report
Mon 27 Feb 2023
SRR3128844.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128844.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2270468
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166710.7342539071239937No Hit
CTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC29750.13103025455544848No Hit
CCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTG28130.12389516170234506No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC24480.10781918089134046No Hit
GCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTG24130.10627764848480578No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT62500.081.042181
AGGGATG49900.078.161755
GAGGGAT30150.074.343674
ATAGGGA22600.073.914993
ATAGCGG4500.072.381581
AGGGCAT20650.072.3642045
TAGGGAT17950.071.768844
AGTAAGG15500.071.569441
TAGGGTA7650.070.320374
ATAGGGC13800.069.7571643
GAATAGG14750.069.447911
AGGGTAC10300.069.3462755
AGATAGG13500.069.234541
TAGAGGG40600.069.1558152
AGGGAAT24200.068.93345
GTAGGGA15500.068.804133
AAGGGTA16600.068.793124
AGTAGGG37700.068.4673842
AAGGGAT39000.068.363074
TAATAGG7750.068.219381
Produced by FastQC (version 0.11.5)