FastQCFastQC Report
Mon 27 Feb 2023
SRR3128843.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128843.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2311697
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163110.7055855503554315No Hit
CTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC31990.13838318776206396No Hit
CCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTG29460.12743884687309798No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC25130.10870801839514435No Hit
GCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTG24900.10771307831432926No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGATG44350.076.404745
CGTTTTT61000.076.279121
ATAGCGG6050.075.431991
AGGGCAT18850.074.29915
CGTAGGG6600.074.094192
TAAGGGA19700.072.763283
GAGGGAT30950.072.432994
AAGGGAT38150.071.94444
AGGGAAT24650.071.4978565
GAATAGG14500.071.058381
ATAGAGG16200.070.86211
ATAGGGC13250.070.230863
AGTAAGG13750.069.459641
ATAGGGA22800.069.26013
ATAAGGG34500.069.237192
AAGGGTA15200.068.95094
AGTAGGG38100.068.619032
GTAGGGC9850.068.2304843
TAGGGCA11050.068.051294
TAGCGGG15200.067.747822
Produced by FastQC (version 0.11.5)