FastQCFastQC Report
Mon 27 Feb 2023
SRR3128842.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128842.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2420871
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT168560.6962783229672296No Hit
CTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC32580.13457966161765744No Hit
CCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTG31430.12982930523766034No Hit
GCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTG27890.11520646907662573No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC24960.10310338716932872No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGATG48900.077.074455
CGTTTTT63300.077.073421
ATAGGGA24400.073.5760353
CGTAGGG6550.073.184942
ATAGCGG5150.073.082311
ATAGAGG16700.070.992681
AAGGGTA14650.070.895314
AGGGAAT24200.070.685595
AGGGTAC11100.070.279725
AAGGGAT39750.070.2282944
AGATAGG14800.069.934341
GATAGGG38200.069.387082
GAATAGG16700.069.020671
GAGGGAT31450.068.7384954
AGTAAGG16500.068.146491
TAGCGGG13950.068.051862
TAGAGGG43550.067.769512
AGGGCAT18350.067.610285
TAAGGGA18950.067.456313
ATAGGGC16300.067.1786353
Produced by FastQC (version 0.11.5)