FastQCFastQC Report
Mon 27 Feb 2023
SRR3128841.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128841.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1646760
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120700.7329544074424931No Hit
CTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGC29740.18059705117928537TruSeq Adapter, Index 22 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTG28940.1757390269377444No Hit
GCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTG26490.16086132769802522No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17000.1032330151327455No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC16870.10244358619349511No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT49050.080.783651
AGGGATG31200.076.212765
AGGGTAC7100.074.129565
AGGGAAT21800.071.351535
AGTAAGG10600.071.202841
ATAAGGG23450.070.380062
AAGGGTA11500.070.116894
TAAGGGA16050.069.630213
GAGGGAT26400.069.481644
TAGAGGG27900.068.957322
ATAGGGA13550.068.904933
CGTAGGG5750.068.886772
AAGGGAT29250.068.515294
GTAGGGA12000.068.374223
TAGGGCA7700.067.9761664
TAGTAGG7050.067.5795751
ACGGGAT5100.067.495854
AGAGGGA41450.067.347513
AAGAGGG58800.067.042992
AGTAGGG27000.066.8897252
Produced by FastQC (version 0.11.5)