FastQCFastQC Report
Mon 27 Feb 2023
SRR3128840.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128840.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1675029
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113710.6788539183500704No Hit
CTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGC31990.19098176807685122TruSeq Adapter, Index 22 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTG29160.17408653820321918No Hit
GCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTG27480.16405686110509132No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC18470.11026674762048896No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17140.10232658658447108No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT42550.078.754721
TAGGGCA8150.073.2351464
AGGGATG33550.072.982485
TAAGGGA16000.072.845913
AAGGGTA13500.072.0626144
ATAAGGG23850.071.581852
TAGAGGG28600.070.546582
AGGGAAT22150.069.806455
GTAGGGA11650.069.790083
AGGGTAC7300.069.5303345
AAGGGAT31800.068.426974
ATAGGGC9550.068.404533
TAGACGG2350.068.093741
AGGGTAT9200.067.941835
CGTAGGG4850.067.879872
ATAGAGG10750.067.860871
ATAGGGA15950.066.5918663
CGAGGGA9950.066.126943
AGTAGGG27500.066.0144962
TAGGGAT12200.065.8733444
Produced by FastQC (version 0.11.5)