FastQCFastQC Report
Mon 27 Feb 2023
SRR3128839.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128839.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1752274
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122330.6981214125188184No Hit
CTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGC34140.1948325433122902TruSeq Adapter, Index 22 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTG31100.17748365837762817No Hit
GCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTG29480.16823852890586746No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC17740.1012398745858239No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT43850.078.4141161
AGGGATG32800.073.357735
AGTAAGG10900.070.340851
AAGGGAT32250.069.5085754
CGTAGGG5550.069.431762
TAAGGGA14950.068.8418663
GAGGGAT25850.068.537864
ATAAGGG24400.068.371622
TATAGGG13000.068.321252
AGTAGGG26650.068.24192
AGGGCAT13250.068.0981145
TAGGGAT11500.067.835964
ATAGGGC9700.067.827533
TAGAGGG32450.067.630022
AAGGGTA12800.067.187874
GTACGGG4700.066.990662
GGGCGAT8700.066.981086
ATAGGGA16700.066.974613
AGTACGG2250.066.8981251
CGAAGGG20450.066.870882
Produced by FastQC (version 0.11.5)