FastQCFastQC Report
Mon 27 Feb 2023
SRR3128837.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128837.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2175929
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT332041.525968907992862No Hit
CCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTG35780.164435512371957TruSeq Adapter, Index 23 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC32200.147982769658385TruSeq Adapter, Index 22 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCT21880.10055475155669141No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT121800.085.3258361
ATAGGGC14300.074.934473
TAGGGCA10700.074.231074
GTAGGGC9300.070.750243
AGGGATG33950.069.355485
GAATAGG14100.068.4016951
AGTAAGG14950.068.288991
AGGGCAT16150.068.09675
TAGAGGG39900.066.586772
ACGGGTA1700.066.350644
ATAAGGG29300.065.960912
GAGGGAT24700.065.835774
ATAGAGG15400.065.6825261
AGTAGGG34800.065.670362
ATAGGGA20900.065.6627963
AAGGGGC70300.065.115814
GATAGGG34900.064.9432452
GTAGGGA18050.064.573943
AGGGAAT21050.064.525085
AAGAGGG82000.064.169492
Produced by FastQC (version 0.11.5)