FastQCFastQC Report
Mon 27 Feb 2023
SRR3128836.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128836.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2256768
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT345801.5322797912767285No Hit
CCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTG37010.16399559015370654TruSeq Adapter, Index 23 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC33940.1503920651125858TruSeq Adapter, Index 22 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT128200.084.980111
ATAGGGC12350.074.2085343
AGGGATG34950.070.9976655
TAGGGCA9500.069.26134
ATAGAGG17050.068.7272951
ATAGGGA20650.067.596253
AGGGCAT18300.067.283335
TAGAGGG42350.067.250542
GTAGGGA15800.067.2260363
GAATAGG13000.066.970371
TAAGGGA19750.066.631133
AGTAAGG13950.066.457861
GATAGGG34250.065.7281652
ATAAGGG32500.065.507562
AGTAGGG39100.065.388092
GAGGGAT27650.065.27134
AGATAGG13000.065.1603551
ACGGGTA1950.065.075174
AGAGGGC35600.064.9533163
TATAGGG18050.064.573012
Produced by FastQC (version 0.11.5)