FastQCFastQC Report
Mon 27 Feb 2023
SRR3128835.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128835.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1783263
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133870.75070250434176No Hit
CTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGC26790.15023022403313477No Hit
CCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTG26020.1459122967279644No Hit
GCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTG21040.11798596168933018No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGGG5950.079.263582
CGTTTTT51200.078.043821
TAGGGCA9050.074.5209054
AGATAGG10000.070.7646561
ATAGCGG3100.070.0037461
ATGCGGG10000.068.384662
ATAGAGG12150.068.3378751
CGAAGGG18100.068.26742
GTAGGGA13750.068.2560353
TATAGGG12550.068.0182652
ACGGGTA1950.067.719554
ATAGGGC9950.067.306333
GAATAGG11950.067.112921
ATAGGGA14200.067.089363
TAGAGGG31900.066.9727552
TACGGGT1550.066.939383
AGGGGCT69650.066.929835
AGGGAAT17450.065.978115
AAGGGAC18000.065.502554
AGAGGGA38400.065.46163
Produced by FastQC (version 0.11.5)