FastQCFastQC Report
Mon 27 Feb 2023
SRR3128834.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128834.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1812150
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130220.7185939353806252No Hit
CTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGC28900.1594790718207654No Hit
CCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTG26700.14733879645724693No Hit
GCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTG22990.12686587754876805No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGGG5300.079.853772
CGTTTTT51050.079.636221
TAGGGCA10200.074.6434864
ATAGAGG11950.071.269431
ATAGGGC10900.071.143393
GTAGGGC9000.068.930043
GAATAGG12500.067.757151
AGTAAGG13550.067.368221
AGGGAAT16500.066.936195
AATAGGG28300.066.798772
AGTAGGG31900.066.18882
AGGGATG24600.065.7204365
ACGGGTA1650.065.512024
AGATAGG12650.065.465851
GGTAAGG9000.065.352191
AAGAGGG66350.065.345962
ATAGGGA14700.065.221373
TAGGGTC5550.065.2040944
TAGAGGG31400.064.846572
CGAAGGG17750.064.3778842
Produced by FastQC (version 0.11.5)