FastQCFastQC Report
Mon 27 Feb 2023
SRR3128833.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128833.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1919031
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133780.6971226624270269No Hit
CTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGC29970.15617256834308565No Hit
CCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTG26850.13991436303009175No Hit
GCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTG24060.12537577558674143No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT53900.079.786941
TAGGGCA10150.076.865164
CGTAGGG5100.071.880642
GTAGGGC7550.070.9651953
AGTAGGG33400.068.9504552
AGATAGG11750.068.8755341
GTAGGGA14700.068.4201353
GAATAGG11800.068.1849441
ATGTAGG6450.067.841841
ATAGGGA15300.067.5800863
ATAGGGC11850.067.027943
AGGGATG25850.066.904015
ATAGAGG14050.065.637821
CGAAGGG18650.065.269222
ATGAGGG30000.065.1717762
GGTAAGG11650.065.024091
AAGGGAC17600.064.8904954
ATGCGGG9200.064.878882
TAGAGGG37200.064.812972
AGGGTAC8750.064.452255
Produced by FastQC (version 0.11.5)