FastQCFastQC Report
Mon 27 Feb 2023
SRR3128832.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128832.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1876566
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116530.6209746952678457No Hit
CTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC35030.1866707592485423No Hit
CCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTG32880.17521366154987353TruSeq Adapter, Index 22 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTG29510.1572553270175416TruSeq Adapter, Index 22 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT50200.077.3824161
AGGGATG27700.072.947385
ATAGGGA16950.072.923683
AGGGAAT20900.069.700595
ATAGGGC12800.069.6608663
AGGGTAT9350.069.356725
AAGGGTA13750.069.3083654
GAGGGAT26200.068.785844
TAGAGGG31750.068.203392
TAGGGCA10050.068.0673754
AAGGGAT32100.068.047624
AGATAGG12050.067.764971
AGTAGGG31900.066.995332
TATAGGG16350.066.943322
AGAGGGC23750.066.545433
TAGGGCG4900.066.4339454
ACGGGTA1850.066.303844
CGTAGGG4700.066.249572
AGTAAGG11800.066.000631
GGTAAGG10600.065.902531
Produced by FastQC (version 0.11.5)