FastQCFastQC Report
Mon 27 Feb 2023
SRR3128831.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128831.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1910708
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116290.6086225629452537No Hit
CTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC37600.19678569409873198No Hit
CCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTG32560.17040803723017856TruSeq Adapter, Index 22 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTG31650.16564540474002307TruSeq Adapter, Index 22 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT49800.077.67661
CGAAGGG18050.074.7672042
AGGGATG28600.074.605175
ATAGGGA15550.071.328113
ACGGGTA1850.071.1320654
TAGAGGG34350.070.499652
AGGGTAT9000.068.408165
AGTAGGG32200.068.197342
GTAGGGC8400.067.6994553
AAGGGAT30950.067.573824
ATAGGGC12050.066.694223
TAAGGGA18300.066.002623
AGTAAGG12350.065.921461
GAGGGAT28200.065.497184
TAGGGCA8900.064.9522554
TAGACGG2900.064.909711
AGGGCAT16300.064.586175
AGAGGGA46500.064.28113
CGTAGGG4100.064.225972
AGATAGG10350.064.110111
Produced by FastQC (version 0.11.5)