FastQCFastQC Report
Mon 27 Feb 2023
SRR3128830.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128830.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1990611
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117520.5903714990020652No Hit
CTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC38420.19300606698144437No Hit
CCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTG35290.1772822515298067TruSeq Adapter, Index 22 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTG34420.172911734135901TruSeq Adapter, Index 22 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT49900.078.259141
GACCGAT950.074.20148
CGAAGGG17750.072.545022
AGGGATG32000.072.253625
ATAGGGA18200.071.009543
TATAGGG16250.069.9872
ATAGGGC15050.069.6343843
TAGGGCA10900.069.413484
AGTAGGG32900.069.2789462
AATGCGG4500.069.0063251
TAGAGGG33150.068.189412
GAGGGAT29500.068.022154
ATGAGGG29800.067.496762
AGATAGG12700.066.314241
ATGCGGG9500.066.288252
AAGGGAT34050.066.247364
GGTAAGG13300.065.798931
AGAGGGC26650.065.599593
AGGGCGA10150.065.2826165
GATAGGG30600.064.810652
Produced by FastQC (version 0.11.5)