FastQCFastQC Report
Mon 27 Feb 2023
SRR3128829.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128829.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2654466
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT209630.7897256924744939No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC37450.14108298994976767No Hit
CCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTG35060.13207929579810027RNA PCR Primer, Index 10 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC34820.1311751591468868TruSeq Adapter, Index 10 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTG27460.10344830184300723RNA PCR Primer, Index 35 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGC14750.079.613423
CGTTTTT81250.077.4538041
AGGGATG46550.073.3913355
GAATAGG15700.073.316291
AGTAGGG41400.072.335632
AGTAAGG17250.071.104091
GAGGGAT36550.069.676344
TAGGGCA12450.069.3203354
TAGAGGG49450.069.2387542
TAAGAGG19200.069.042331
TAGGGCG4850.069.039174
GTAGGGC12050.068.881813
ATAACGG2400.068.796621
AAGGGAT45450.068.587744
AGGGAAT23750.068.460895
ATAGAGG18550.068.409851
ATAGGGA22950.068.018063
AGAGGGA62100.067.892983
ACGGGAT6500.067.4758764
TAAGGGA22300.067.462863
Produced by FastQC (version 0.11.5)