FastQCFastQC Report
Mon 27 Feb 2023
SRR3128828.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128828.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2694572
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200380.7436431462955898No Hit
CTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC38860.1442158532041452TruSeq Adapter, Index 10 (95% over 23bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC38220.1418407079120543No Hit
CCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTG36600.1358286213914492RNA PCR Primer, Index 10 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTG28750.10669597991814656RNA PCR Primer, Index 35 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT77650.077.610241
ATAGGGC17600.074.768973
GAATAGG16350.073.948061
AGGGATG44100.072.574585
AGTAGGG43700.070.687942
TAGGGCA13750.070.06924
CGTAGGG8850.069.5968252
AGATAGG17100.068.503791
TAGAGGG49600.068.2514952
GGTAAGG14800.068.02421
TATAGGG21250.067.926742
ATAGGGA24050.067.418653
AGGGAAT24500.067.139485
GAGGGAT34600.067.100674
TAGGGTC7100.066.855794
TAAGGGA23900.066.661923
GTAGGGA20600.066.389863
AATAGGG41200.065.961722
TAGGGTA7450.065.607434
AGGGTAC12250.065.6048665
Produced by FastQC (version 0.11.5)