FastQCFastQC Report
Mon 27 Feb 2023
SRR3128827.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128827.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2808373
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210010.7477995266298316No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC39090.13919091231827113No Hit
CTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC39050.13904848109563794TruSeq Adapter, Index 10 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTG37950.1351316224732256RNA PCR Primer, Index 10 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTG29440.10482937985801742RNA PCR Primer, Index 35 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT82350.077.367091
ATAGGGC16850.075.308963
TAGGGCA13000.075.196054
GAATAGG16100.072.189211
ATAGCGG5850.070.7828451
ATAGGGA24150.070.643523
AGTAGGG45150.070.574362
AGGGATG45850.070.4194265
GAGGGAT36250.070.139774
TAAGGGA24350.069.291233
TAGAGGG49650.067.774892
AGAGGGC40900.067.5673453
TAAGAGG20550.067.318851
AAGAGGG107900.066.902882
AGTAAGG19450.066.045651
GGTAAGG18250.066.0052261
AATAGGG42400.065.2866742
TATAGGG20900.065.211882
AGATAGG15350.064.987331
AGGGTAC14250.064.972035
Produced by FastQC (version 0.11.5)