FastQCFastQC Report
Mon 27 Feb 2023
SRR3128826.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128826.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2378809
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT229540.9649366552758123No Hit
CCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTG25780.10837356004622482RNA PCR Primer, Index 16 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT90400.081.709691
TAGGGCA12250.075.830324
ATAGGGC13150.073.509063
AGTAGGG40200.070.143572
GAATAGG15500.069.991661
CGTAGGG6350.069.801842
AGGGATG34500.069.331425
ATAAGGG35800.067.305512
ATAGGGA20300.067.129683
TAGAGGG43350.066.787012
ACGGGTA2200.066.443394
TATAGGG19150.066.236332
GTAGGGC10650.065.970443
GTACGGG6800.065.876042
TAGGGCG5800.065.852124
AAGGGGC82750.065.5303654
AGGGAAT21400.065.438595
GGTAAGG13350.065.36431
AATAGGG34700.065.226562
AGACGGG17150.064.337552
Produced by FastQC (version 0.11.5)