FastQCFastQC Report
Mon 27 Feb 2023
SRR3128825.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128825.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2436267
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT223420.9170587624427043No Hit
CCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTG27420.11254924029262803RNA PCR Primer, Index 16 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT89750.080.810211
ATAGGGC13850.076.688873
CGTAGGG7200.071.202882
ATAGGGA20700.069.9284743
AGTAGGG38350.069.169932
GAATAGG13750.068.458181
AGGGATG36750.067.778545
TAGAGGG47450.067.6999052
ATAGCGG4800.067.655941
ATAGAGG18850.067.1643751
TAGGGCA13400.066.98884
AAGGGGC78950.065.957064
TAGGGAT14500.065.472214
ACGGGAT7000.065.124954
AGAGGGC37700.065.0732963
AAGAGGG91500.064.9724962
AATAGGG37250.064.899362
GTACGGG6200.064.480862
TATAGGG21400.064.1762
ATAAGGG36450.064.130172
Produced by FastQC (version 0.11.5)