FastQCFastQC Report
Mon 27 Feb 2023
SRR3128824.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128824.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2557310
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT229720.8982876538237445No Hit
CCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTG28600.11183626545080573RNA PCR Primer, Index 16 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC26070.10194305735323445RNA PCR Primer, Index 16 (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT92850.083.325331
ATAGGGC13600.072.5690163
GAATAGG15200.070.590851
TAGGGTA7100.069.5027164
AGTAGGG39800.069.431522
TAGAGGG46250.069.198662
ATAGGGA21300.069.061443
TAGGGCA12550.068.904084
GTACGGG6150.068.0107042
CGTAGGG7350.067.776772
ATAGCGG5650.067.4673541
ATAGAGG17800.067.153841
AGGGATG34450.066.300675
AGAGGGC39300.065.532823
AAGAGGG91900.065.508252
AGGGCAT19300.065.50375
AGGGAAT22350.064.975825
GATAGGG38000.064.805232
AAGGGAT36200.064.6534354
ATAACGG1900.064.3986741
Produced by FastQC (version 0.11.5)