FastQCFastQC Report
Mon 27 Feb 2023
SRR3128823.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128823.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1769821
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115900.6548684867000675No Hit
CTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC38210.21589753992070385No Hit
CCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTG28440.16069421709879134No Hit
GCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTG25410.1435738416483927No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT48100.078.47931
AGGGATG25150.075.8604665
ATAGCGG3000.072.351381
TAGGGTA6000.072.318564
CGTAGGG4850.071.961992
AGTAAGG10650.070.889281
ATAGGGC10150.070.630243
ATAGAGG12000.069.5988851
AGGGCAT16300.069.1912465
GGTAAGG8750.069.025921
ATAGGGA14700.068.981763
GAATAGG11850.068.887111
TAGAGGG31450.068.534422
TAAGGGA14650.068.251373
AGTACGG2700.068.157091
ACGGGAT4850.068.072154
AAGGGAT33250.067.944924
GTACGGG4200.067.377542
AGGGAAT18050.066.908835
ACGGGTA1850.066.284934
Produced by FastQC (version 0.11.5)