FastQCFastQC Report
Mon 27 Feb 2023
SRR3128822.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128822.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1797680
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110050.6121779182056873No Hit
CTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC40990.22801610965244093No Hit
CCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTG30870.1717213297138534No Hit
GCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTG28120.1564238351653242No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGGG5200.078.682322
CGTTTTT47900.077.511971
ACGGGAT6300.073.1086654
ATAGGGC10300.073.007273
AGGGATG24350.071.8004765
GTAGGGT7550.070.9645163
GGTAAGG9150.069.943131
TAGAGGG33100.068.908852
GAATAGG11500.068.744621
AGGGTAC7100.067.518875
AGTAGGG28650.067.136692
TAGGGCA9450.066.643274
ATAAGGG26650.066.528142
AGTAAGG10850.066.357341
GAGGGAT19350.066.307864
TATAGGG13000.066.201682
TAAGGGA15350.066.13463
TAGGGAC8400.066.02164
ATAGCGG3300.065.595061
AAGGGAT31850.065.517434
Produced by FastQC (version 0.11.5)