FastQCFastQC Report
Mon 27 Feb 2023
SRR3128820.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128820.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1785668
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122940.688481845449434No Hit
CTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTGC34460.1929810020675736No Hit
CCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTG30570.17119643741165771No Hit
GCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTG29550.16548428935278003No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT48100.080.697681
CGTAGGG4450.073.1012042
GTAGGGC8050.072.6208343
AGTAGGG31850.070.0143662
AGGGATG24450.069.38465
TAGAGGG31850.068.978212
AAGGGAT29250.068.501254
GAGGGAT22300.068.2862244
ATAGCGG2350.068.2365951
GGTAAGG10100.068.176981
AGGGCAT15000.067.043595
ATAAGGG24350.066.022332
AAGGGTC13950.065.901594
GAATAGG10450.065.4422151
ATAGGGA17650.065.442043
AAGAGGG60250.065.41612
AGAGGGA42900.065.387563
TAAGGGA16550.065.233843
AAGGGCA28550.065.226854
AGTAAGG12950.065.1912841
Produced by FastQC (version 0.11.5)