FastQCFastQC Report
Mon 27 Feb 2023
SRR3128819.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128819.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1846119
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122330.662633340537636No Hit
CTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTGC37250.20177464182969787No Hit
CCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTG33000.17875337396993368No Hit
GCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTG31730.17187407745654532No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT49800.081.0663451
CGTAGGG4050.078.958912
AGGGCAT14800.070.4965445
TAGGGCA8000.069.909074
ACGGGAT4000.069.32164
AAGGGAT30000.069.243284
AGTAGGG33350.068.1080552
AGGGATT20550.067.9236765
AAGGGCG8450.067.854674
TAGAGGG32800.067.529612
TAAGGGA16000.067.265463
AAGAGGG63950.067.13942
AGTAAGG12000.066.657711
GGTAAGG9600.066.65771
AAGGGTA13650.066.450964
TAGGGTC6350.065.870794
GTAGGGC7650.065.7353443
AGGGATG25150.065.591225
AGGGTAC10050.065.469435
ATAAGGG25150.065.258132
Produced by FastQC (version 0.11.5)