FastQCFastQC Report
Mon 27 Feb 2023
SRR3128818.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128818.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1932749
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123080.636813160943299No Hit
CTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTGC36340.18802234537438645No Hit
CCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTG33780.17477696276133114No Hit
GCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTG31900.16504988490486866No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT50350.079.88651
CGTAGGG5600.076.3678362
GGTAAGG10750.073.520351
ATAGCGG3150.073.1799851
ACGGGAT5100.072.797084
TAGGGTA5650.071.533114
AGTAGGG32550.071.323562
TAGGGCA7950.070.936744
TAGAGGG34050.070.5278852
ATAGGGT9750.069.408873
ATAAGGG25400.069.013212
AAGGGTA13000.068.3243564
AAGGGAT30750.068.009874
ATAGGGC9500.067.772593
AAGAGGG67050.067.707292
GAATAGG11700.067.550761
AGGGTAT10650.067.513045
AGGGAAT18150.067.0608445
TATAGGG15850.067.009482
ATAGGGA16600.066.8133
Produced by FastQC (version 0.11.5)