FastQCFastQC Report
Mon 27 Feb 2023
SRR3128817.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128817.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2442874
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT276511.1319044698989797No Hit
CCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTG36070.14765395186161873RNA PCR Primer, Index 10 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC32680.13377685463924868TruSeq Adapter, Index 10 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT119300.083.157851
ATAGGGC13950.076.054583
AGTAGGG39350.071.6594542
AGGGATG34550.071.1350565
ATAGGGA22450.070.363193
AATAGGG34700.068.896252
GAATAGG14050.068.8306661
TATAGGG17850.068.6834642
TAGAGGG45050.068.3495252
TAGGGCA13500.068.11114
CGTAGGG6450.067.258192
AGTAAGG14850.067.028641
AGAGGGC38600.066.4551543
AAGAGGG93750.066.442912
TAAGGGA20300.065.968923
TAAGAGG18750.065.918061
AAGGGTA15550.065.499874
GAGGGAT32400.065.491444
ATAGCGG3900.065.318111
AGAGGGA58150.065.115273
Produced by FastQC (version 0.11.5)