FastQCFastQC Report
Mon 27 Feb 2023
SRR3128816.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128816.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2520142
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT271211.0761695174319543No Hit
CCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTG39270.15582455274345652RNA PCR Primer, Index 10 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC36750.14582511620376948TruSeq Adapter, Index 10 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT115650.082.9803541
ATAGCGG4200.071.683871
AGGGATG34650.071.61565
AGTAGGG39900.070.702752
ATAGGGC16000.069.9091343
GTAGGGT8000.069.9091343
CGTAGGG7150.069.704012
GTACGGG6350.069.600462
TAGAGGG46600.068.709872
GTAGGGC10050.067.807683
ATAGGGA23000.067.635873
TAGGGCA14750.067.549294
AAGGGAT39250.067.1738364
AGTAAGG16450.066.9176561
AAGAGGG92950.066.719582
ATAAGGG32650.066.385882
AGTACGG3550.066.25711
GAATAGG14250.065.6944961
TATAGGG18800.065.2740552
AAGGGGC78550.065.096814
Produced by FastQC (version 0.11.5)