FastQCFastQC Report
Mon 27 Feb 2023
SRR3128815.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128815.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2639315
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT279651.059555225503587No Hit
CCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTG41160.1559495550928934RNA PCR Primer, Index 10 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC38490.14583329386602206TruSeq Adapter, Index 10 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT124100.083.7806551
ATAGGGC14900.075.069093
TAAGAGG20000.069.15771
AGGGATG35700.068.717065
AAGAGGG99650.068.289432
AGTAAGG15300.067.9554141
GAATAGG15900.066.87051
TATAGGG21250.066.789822
AGTAGGG42150.066.675422
TAAGGGA22600.066.544473
AGAGGGC41750.066.302413
TAGAGGG46850.066.205892
ACGGGTA3200.066.0883264
ATAGGGA23700.066.0338063
TGTAGGG17350.065.8216862
TAGGGCA13700.065.520174
AATAGGG38650.065.296072
CGTAGGG8600.065.029552
GTACGGG6050.063.6972582
AGAGGGA60350.063.623163
Produced by FastQC (version 0.11.5)