FastQCFastQC Report
Mon 27 Feb 2023
SRR3128814.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128814.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2290617
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT233681.0201618166633706No Hit
CCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTG26490.11564569720734631No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT100150.083.219521
ACGGGTA3150.074.842254
GAATAGG15200.074.474441
AGGGATG36150.072.797135
CGTAGGG5550.072.2126542
ATAGCGG4300.070.202261
ATAACGG2050.069.0250851
TAGGGCA12800.068.88414
CGAAGGG23500.068.819842
TATAGGG18200.068.653372
AGGGCGA15800.068.110325
TAGAGGG39650.067.663812
GAGGGAT31800.067.6122064
AGAGGGC34350.067.259973
ATAGGGC15000.066.953883
AGGGTAC10850.066.699945
AGGGCAT20850.066.4891055
AAGAGGG79100.066.3446662
ATAAGGG30800.066.286422
AGTAGGG38750.066.1933752
Produced by FastQC (version 0.11.5)