FastQCFastQC Report
Mon 27 Feb 2023
SRR3128812.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128812.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2525581
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT238720.9452082510915311No Hit
CCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTG28790.11399357217210615No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT105050.083.5228651
CGTAGGG7950.073.300012
AGGGATG41550.071.3672945
ATAGGGC15750.071.312683
AGTAGGG41950.071.2483142
ATAGAGG16950.069.666921
GAATAGG17050.068.982391
GTAGGGA19950.068.077633
TAGAGGG44500.067.0599442
AGGGCAT21450.065.9447255
AGGGCGA17900.065.8963855
GAGGGAT33100.065.876554
AGGGTAC10150.065.745065
TAGGGTA8150.065.73374
ATAGGGA25700.065.463523
TAGGGAT16600.065.111944
TAGGGCA12800.064.9836654
AAGAGGG93100.064.914352
GGTAAGG14250.064.378731
ATAGCGG4700.064.062631
Produced by FastQC (version 0.11.5)