FastQCFastQC Report
Mon 27 Feb 2023
SRR3128811.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128811.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2357338
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175830.7458837044157435No Hit
CCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTG31920.13540697176221653No Hit
CTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC31130.13205573405256268TruSeq Adapter, Index 14 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT70400.079.738051
CGTAGGG6050.074.8303452
ATAGCGG3600.072.0694961
AGTAGGG38250.070.029142
TAGAGGG41200.069.936822
GTAGGGC9900.069.547193
ATAAGGG31600.068.797982
AAGGGGC83450.068.717784
GAATAGG14150.068.00881
ATAGGGC12650.066.73053
AGTAAGG14000.066.715771
TAGGGCA10950.066.3236544
AAGAGGG85500.066.13252
AGGGGCT96200.065.848345
AGAGGGC34550.065.7901463
AATAGGG34950.065.307052
AGGGAAT21000.064.894635
ATAGAGG15850.064.285911
GGATAGG12700.064.2589651
ATGAGGG40250.064.206142
Produced by FastQC (version 0.11.5)