FastQCFastQC Report
Mon 27 Feb 2023
SRR3128810.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128810.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2402300
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170810.7110269325229988No Hit
CTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC34050.1417391666319777TruSeq Adapter, Index 14 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTG33110.1378262498438996No Hit
GCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTG25650.1067726761853224No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT70550.078.592151
ATAGGGC11150.075.8712543
CGTAGGG6450.072.195112
AGTAGGG39350.070.2853552
TAGAGGG44300.069.22712
GAATAGG15450.069.155751
TAGGGCA11400.068.435724
AGGGGCT94450.068.021495
AGTAAGG15750.067.83851
ATGTAGG8450.067.399941
ATAGCGG3300.067.037041
ATAAGGG32350.066.737612
AAGGGGC85350.066.683784
AATAGGG35650.065.837492
AGGGAAT22250.065.691615
AAGAGGG90750.065.4101262
TAGTAGG10150.065.385881
AGAGGGA55050.065.31063
GTAAGGG36600.065.02822
AGGGCAT19900.064.7108465
Produced by FastQC (version 0.11.5)