FastQCFastQC Report
Mon 27 Feb 2023
SRR3128809.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128809.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2534041
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT176980.6984101677912867No Hit
CTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC34760.13717220834232755TruSeq Adapter, Index 14 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTG34000.13417304613461267No Hit
GCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTG27580.10883801801154756No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT75750.077.633391
ATAGCGG4650.071.833431
ATAGGGC13500.070.31953
AGTAGGG42700.069.888232
TAGGGTA6700.068.739994
ATAGGGT10000.068.1437763
TAGAGGG47150.067.578132
AAGGGGC85800.067.535794
GAATAGG16150.067.291571
TAGGGCA11050.066.772194
AAGAGGG93000.066.653052
ATAAGGG34650.066.594212
AGGGAAT21550.066.513655
ATAGAGG17500.066.401841
ATGAGGG42150.066.117332
AATAGGG37900.066.091592
AGTAAGG15500.065.8641741
GTACGGG5150.065.7027362
CGTAGGG6550.065.291752
GTAGGGC11200.065.0387043
Produced by FastQC (version 0.11.5)