FastQCFastQC Report
Mon 27 Feb 2023
SRR3128808.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128808.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2542946
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT182090.7160592478172954No Hit
CCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTG39860.15674733163818658No Hit
CTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGC39550.155528273113153No Hit
GCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTG28620.11254662898858253No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT77850.079.941991
TAGGGCA12350.076.004224
AGGGATG45100.075.854425
ATAGGGC14550.075.5343553
ACGGGTA2600.074.381014
AGTAAGG17100.072.844471
ATAGAGG18350.070.967871
GGTAAGG14750.070.695051
GTAGGGC12550.070.2828753
TAAGGGA21050.069.464233
AGTAGGG43700.069.079652
GTAGGGT10600.068.972733
AGATAGG14000.068.078831
AAGGGCG11450.067.972014
AAGGGTA19200.067.804654
GGATAGG15750.067.704361
CGTAGGG6500.067.487172
AGGGTAC12400.067.456595
TAGAGGG48500.066.9111252
TGTAGGG18650.066.263462
Produced by FastQC (version 0.11.5)