FastQCFastQC Report
Mon 27 Feb 2023
SRR3128807.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128807.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2660314
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180790.6795814328684509No Hit
CCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTG44490.16723589771733713No Hit
CTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGC42490.15971798817733546No Hit
GCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTG30320.1139715086264253No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT78000.079.264781
TAGGGCA11100.076.636714
ATAGGGC15250.075.197133
AGGGATG46250.075.095515
AGTAAGG19000.074.5405041
ATAGGGA23050.071.771673
ATAGAGG19200.070.333161
ATAACGG2950.070.179481
TAAGGGA21200.069.3888553
TAGAGGG52200.069.277192
AGATAGG14200.068.590111
GAATAGG17200.068.1161961
GTAGGGC11050.068.05173
AGTAGGG43900.067.5926062
ACGGGTA3350.067.340714
GGTAAGG14850.067.172091
AAGGGTA22300.066.598354
AGAGGGC39350.066.406613
AATAGGG41450.066.2556152
TAGGGAT15950.066.0037464
Produced by FastQC (version 0.11.5)