FastQCFastQC Report
Mon 27 Feb 2023
SRR3128806.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128806.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2828715
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT184710.6529820077314258No Hit
CCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTG45540.16099182844507134No Hit
CTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGC45160.1596484622876465No Hit
GCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTG33060.11687285569596088No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT83000.079.589911
AGGGATG46250.074.077375
AGTAAGG18950.073.990521
TAGGGCA11550.073.242014
TAAGGGA21300.072.812193
ATAGGGC14600.072.426823
GGTAAGG17150.071.330991
ATAGCGG6450.070.7588961
CGTAGGG8700.069.6839142
TAGAGGG54450.068.789562
ATAGAGG21250.068.19631
GTAGGGT11250.068.093373
AAGGGTA21300.067.958044
GAGGGTA14700.067.777924
AGGGTAC12700.067.3499765
GTAAGGG47300.066.9669952
AGTAGGG48300.066.845432
ACGGGTA3450.066.74934
GAGGGAT34300.066.17944
ATAGGGA21600.066.143873
Produced by FastQC (version 0.11.5)