FastQCFastQC Report
Mon 27 Feb 2023
SRR3128805.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128805.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences527289
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33350.6324804803437962No Hit
CCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTG21410.40603919292835616RNA PCR Primer, Index 34 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC18160.3444031641092456RNA PCR Primer, Index 34 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTG17760.3368171913315089TruSeq Adapter, Index 18 (95% over 22bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC14270.27062957884575634No Hit
AGCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCT7430.14090944434645897RNA PCR Primer, Index 34 (95% over 23bp)
AAAGCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTT6560.12440995355488167RNA PCR Primer, Index 34 (95% over 21bp)
AGAGCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTT6130.11625503281881473RNA PCR Primer, Index 34 (95% over 21bp)
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC5760.1092380079994083No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGAT254.9221126E-793.985118
CGTAGGG1700.086.0104142
CGTTTTT16500.085.194571
ATCGGGT401.0059011E-982.542253
CGGGAAT1200.082.236985
AGGGATG13650.081.247215
AAGGGAC5400.081.2320564
ATAGCGG1400.080.865411
CTAACGG353.7642167E-880.865411
CGAAGGG3500.080.857712
AATCGGG650.079.8210752
AAGGGTG3550.079.718874
TAGCGGG2250.079.659822
GGGCGAT1600.079.2999346
CGCTACG2300.077.9280853
TAAGGGA5750.077.9280853
CGAGGGA3800.076.956683
AGGGAAT9050.076.849715
GGATGAC7950.076.843177
TAGGGCG800.076.646374
Produced by FastQC (version 0.11.5)