FastQCFastQC Report
Mon 27 Feb 2023
SRR3128804.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128804.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences541403
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32320.5969675084918259No Hit
CCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTG22720.41965042676158054RNA PCR Primer, Index 34 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTG19070.35223299464539354TruSeq Adapter, Index 18 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC18520.3420742035045982RNA PCR Primer, Index 34 (96% over 25bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC14150.2613579902586428No Hit
AGCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCT7800.1440701289058243RNA PCR Primer, Index 34 (95% over 23bp)
AAAGCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTT6600.12190549368954365RNA PCR Primer, Index 34 (95% over 21bp)
AGAGCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTT6580.12153608310260564RNA PCR Primer, Index 34 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTG6200.11451728195078342TruSeq Adapter, Index 3 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCT5680.10491260669039514RNA PCR Primer, Index 34 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATCGC201.8347839E-594.0230194
GGTACGG254.918602E-793.996957
GATGCGG700.087.35551
GTAGCGG1100.085.522861
GGACCGA550.085.4517757
GCATAGG1050.085.115611
ATAGCGG950.084.172491
CGTTTTT16350.083.142861
AGGGTAT3450.083.0987555
AGGGATG14550.081.076415
AGGGTAC1700.080.1738745
CGAGGGA3650.079.833033
TAGAGGG8800.079.606382
GGGTACG950.079.155336
CGGGAAT950.079.155335
TAAGGGA5600.078.051043
GGATGAC7650.077.4092567
GTCACGG551.8189894E-1276.970571
CGAAGGG2750.076.935022
CGGGTAT551.8189894E-1276.90665
Produced by FastQC (version 0.11.5)