FastQCFastQC Report
Mon 27 Feb 2023
SRR3128803.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128803.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences569706
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33230.5832833075305508No Hit
CCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTG24630.43232825352023674RNA PCR Primer, Index 34 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC20850.3659782414087266RNA PCR Primer, Index 34 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTG20350.35720178478021997TruSeq Adapter, Index 18 (95% over 22bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC15420.2706659224231446No Hit
AGCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCT8210.1441094178400789RNA PCR Primer, Index 34 (95% over 23bp)
AAAGCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTT7160.125678858920215RNA PCR Primer, Index 34 (95% over 21bp)
AGAGCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTT7010.12304592193166299RNA PCR Primer, Index 34 (95% over 21bp)
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC6510.11426946530315636No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGG409.094947E-1294.074681
GACCGAT156.905126E-493.983858
CGTAGGG1550.090.960092
ATAGCGG1250.086.5487061
CGGGTAC550.085.4398655
TACGAGG2050.084.896651
GCATAGG900.083.621931
GGTCGAA353.8657163E-880.5575947
AGGGATG16450.080.271935
CGTTTTT16200.080.137691
TAGGGCA3300.079.750874
AAGGGAC5750.079.28034
GGATGGC13050.079.22017
TAGCGGG2800.078.886232
AGGGCAT4950.078.794545
GTAGGGC2750.078.611583
AGCGGGA4200.078.326753
CGAGGGA5400.078.326753
GGCGTAC301.4566831E-678.319887
GCACCGA600.078.319888
Produced by FastQC (version 0.11.5)